Composite

Part:BBa_K4949009:Design

Designed by: UManitoba_2023   Group: iGEM23_UManitoba   (2023-10-11)


lpp-OmpA-Est119


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been codon optimized for use in E.coli and expresses well on the cell surface.


Source

Est119 is an esterase originally identified in the Thermobifida alba strain AHK119.

References